Hrvatske vode
 
Citostolske koncentracije metala u jetri klena – potencijalni pokazatelj izloženosti metalima u riječnoj vodi
Cytosolic concentrations of metals in chub liver – potential indicators of exposure to metals in river water

Zrinka Dragun1*, Nesrete Krasnići1, Biserka Raspor1

Sažetak/Abstract: 

Uredba o standardu kakvoće voda (NN 89/2010) zahtjeva održavanje dobrog kemijskog statusa prirodnih voda, što uključuje definiranje izvora onečišćenja, kao i redovno praćenje koncentracija prioritetnih toksičnih tvari u vodi, u koje se ubrajaju i spojevi nekoliko metala (Cd, Pb, Ni i Hg). Zbog smanjene mogućnosti razrjeđenja unešenih toksičnih tvari, mali vodotoci su posebno osjetljivi na onečišćenje. Istraživanje provedeno na takvom vodotoku, rijeci Sutli, pokazalo je da su unatoč umjerenom antropogenom utjecaju koncentracije otopljenih metala Fe, Mn i Cd na pojedinim postajama u ovoj rijeci bile uočljivo povišene, što je vidljivo iz širokog raspona njihovih koncentracija (Fe: 3,1-80,5 µg L-1; Mn: 0,44-261,1 µg L-1; Cd: 6-308 ng L-1). Nasuprot tome, koncentracije otopljenog Zn i Cu bile su niske duž cijelog riječnog toka (Zn: <5 µg L-1; Cu: 0,17-3,74 µg L-1). Integrirani pristup procjeni onečišćenja riječne vode metalima zahtjeva povezivanje podataka o koncentracijama otopljenih metala u vodi s podacima o akumulaciji metala u ciljnim tkivima indikatorskih organizama. U citosolu jetre klena (Squalius cephalus) utvrđene su povišene koncentracije Fe, Mn i Cd - istih metala koji su u povišenim koncentracijama nađeni i u vodi rijeke Sutle, što ukazuje na mogućnost primjene citosolskih koncentracija metala u jetri klena u istraživačkom monitoringu kao pokazatelja povećane biološke raspoloživosti metala u riječnoj vodi.

 

The Regulation on Water Quality Standard (OG 89/2010) requires maintenance of good chemical status of natural waters, which includes determining sources of pollution as well as regular monitoring of concentrations of priority toxic substances in water, such as compounds of several metals (Cd, Pb, Ni and Hg). Due to a reduced dissolution potential of toxic matter which entered into water, small watercourses are particularly sensitive to pollution. An investigation carried out on such watercourse, the Sutla River, revealed that, regardless of a moderate anthropogenic impact, concentrations of dissolved Fe, Mn and Cd at some stations in the river were visibly increased, as seen from the wide ranges of their concentrations (Fe: 3.1-80.5 µg L-1; Mn: 0.44-261.1 µg L-1; Cd: 6-308 ng L-1). Conversely, the concentrations of dissolved Zn and Cu were low along the entire river flow (Zn: <5 µg L-1; Cu: 0.17-3.74 µg L-1). Integrated approach to estimation of river water pollution by metals requires linking of data on concentrations of dissolved metals in water with data on accumulation of metals in target tissues of indicator organisms. In the cytosol of chub liver (Squalius cephalus), increased concentrations of Fe, Mn and Cd were determined, i.e. the same metals which were found in increased concentrations in the water of the Sutla River. This indicates the possibility of applying cytosolic concentrations of metals in chub liver in investigative monitoring as indicators of increased biological availability of metals in river water.

Kategorija: 
Izlaganje (referat) sa znanstvenog skupa / Conference Paper
Ključne riječi/Key words: 

citosolske koncentracije Cd, Cu, Zn, Mn i Fe, jetra, klen, metali otopljeni u vodi, rijeka Sutla

cytosolic concentrations of Cd, Cu, Zn, Mn and Fe; liver; chub; metals dissolved in water; Sutla River

Podaci o autorima/Authors affiliations: 

1Institut „Ruđer Bošković“, Zavod za istraživanje mora i okoliša, Bijenička 54, 10000 Zagreb, zdragun@irb.hr *

Literatura/References: 

Andres, S., Ribeyre, F., Tourencq, J.-N., Boudou, A. (2000.): Interspecific comparison of cadmium and zinc contamination in the organs of four fish species along a polymetallic pollution gradient (Lot River, France). The Science of the Total Environment, 248, 11-25.

 

Crane, M., Kwok, K.W.H., Wells, C., Whitehouse, P., Lui, G.C.S. (2007.): Use of field data to support European Water Framework Directive quality standards for dissolved metals. Environmental Science & Technology, 41, 5014-5021.

 

Dragun, Z., Roje, V., Mikac, N., Raspor, B. (2009.): Preliminary assessment of total dissolved trace metal concentrations in Sava River water. Environmental Monitoring and Assessment, 159, 99-110.

 

Dragun, Z., Kapetanović, D., Raspor, B., Teskeredžić, E. (2011.): Water quality of medium size watercourse under baseflow conditions: The case study of river Sutla in Croatia. AMBIO – A Journal of Human Environment, DOI 10.1007/s13280-010-0119-z.

 

European Parliament and the Council of the European Union (EPCEU) (2008.): Directive 2008/105/EC of the European Parliament and of the Council on environmental quality standards in the field of water policy, amending and subsequently repealing Council Directives 82/176/EEC, 83/513/EEC, 84/156/EEC, 84/491/EEC, 86/280/EEC, and amending Directive 2000/60/EC of the European Parliament and of the Council.Official J L 348/84.

 

Fidan, A.F., Ciğerci, İ.H., Konuk, M., Küçükkurt, İ., Aslan, R., Dündar, Y. (2008.): Determination of some heavy metal levels and oxidative status in Carassius carassius L., 1758 from Eber Lake. Environmental Monitoring and Assessment, 147, 35-41.

 

Gaillardet, J., Viers, J., Dupré, B. (2004.): Trace elements in river waters. Treatise on geochemistry: Surface and ground water, weathering, and soils (vol. 5), (ur. H.D. Holland i K. K. Turekian), 225-272, Elsevier, Amsterdam.

 

Giguère, A., Campbell, P.G.C., Hare, L., McDonald, D.G., Rasmussen, J.B. (2004.): Influence of lake chemistry and fish age on Cd, Cu and Zn concentrations in various organs of indigenous yellow perch (Perca flavescens). Canadian Journal of Fisheries and Aquatic Sciences, 61, 1702-1716.

 

Harte, J., Holdren, C., Schneider, R., Shirley, C. (1991.): Toxics A to Z, a guide to everyday pollution hazards. Oxford, Engleska, University of California Press.

 

Holcombe, G.W., Benoit, D.A., Leonard, E.N., McKim, J.M. (1976.): Longterm effects of lead exposure on three generations of brook trout. Salvalinus fontinalis. Journal of the Fisheries Research Board of Canada, 33, 1731-1741.

 

NN (2010.): Uredba o standardu kakvoće voda. Narodne novine, 89.

 

Podrug, M., Raspor, B., Erk, M., Dragun, Z. (2009.): Protein and metal concentrations in two fractions of hepatic cytosol of the European chub (Squalius cephalus L.). Chemosphere, 75, 843-849.

 

Robson, A.J., Neal, C. (1997.): Regional water quality of the river Tweed. Science of the Total Environment, 194/195, 173-192.