Hrvatske vode
 
Perzistencija koliformnih bakterija u mikrokozmosima izvorske vode
Persistence of coliform bacteria in spring water microcosmos

Doris Iličić1, Goran Palijan2

Sažetak/Abstract: 

Planina Papuk, geološki vrijedno područje Republike Hrvatske, u svojim šumama skriva brojne prirodne izvore. Kako bi odredili kvalitetu odabrane izvorske vode, proveli smo istraživanje u kojem smo određivali prisustvo koliformnih bakterija kao glavnih indikatora zagađenja vode. Također, ispitivali smo mogućnost preživljavanja namjerno dodanih koliforma u mikrokozmose s kvarcnim pijeskom u laboratorijskim uvjetima. Nakon prikupljanja i analiziranja uzoraka s četiri odabrana izvora (Jankovac, Kokočak (Vučenović), Muška voda i Žervanjska) rezultati su pokazali kako voda s izvora Kokočak (Vučenović) nije prikladna za piće zbog povećanog ukupnog broja mikroorganizama te koliformnih bakterija, dok su se ostala ispitana izvorišta pokazala prikladna za ljudsku upotrebu. Nadalje, izvor Muška voda pokazao se kao izvor s najvećom kvalitetom vode, kako zbog ispitivanih parametara, tako i zbog nemogućnosti preživljavanja inokuliranih koliforma koji su u mikrokozmosima s ostalih izvora perzistirali. Kako bi potvrdili rezultate, ponovili smo istraživanje na izvoru Muška voda te su isti ovoga puta pokazali mogućnost perzistencije inokuliranih koliforma kao i veći porast aerobnih mezofilnih bakterija u mikrokozmičkim uvjetima.

 

Mount Papuk is a geologically valuable area of the Republic of Croatia, with numerous natural springs hidden in its forests. In order to determine the quality of selected spring water, we carried out an investigation where we were determining the presence of coliform bacteria as the main indicators of water pollution. Additionally, we examined the survival possibility of deliberately added coliforms into the microcosms with quartz sand in laboratory conditions. After collecting and analysing samples from four selected springs (Jankovac, Kokočak (Vučenović), Muška voda and Žervanjska), the results showed that water from the spring Kokočak (Vučenović) was unsuitable for drinking due to increased total number of microorganisms and coliform bacteria, whereas the other tested springs were shown as suitable for human use. Furthermore, the spring Muška voda proved to be the spring with the highest quality of water, both with respect to tested parameters and survival inability of inoculated coliforms that persisted in the microcosms of the other springs. In order to verify the results, we repeated the investigation of the spring Muška voda. This time, the persistence of inoculated coliforms, including a larger increase in aerobic mesophilic bacteria in microcosmic condition, proved possible.

Kategorija: 
Izvorni (originalni) znanstveni članak / Original Scientific Paper
Ključne riječi/Key words: 

izvorska voda, bakterije, biofilm, mikrokozmos, Papuk

spring water, bacteria, biofilm, microcosm, Papuk

Podaci o autorima/Authors affiliations: 

1Sveučilište u Zagrebu, Prirodoslovno-matematički fakultet, Biološki odsjek, Rooseveltov trg 6, 10000 Zagreb, dilicic@stud.biol.pmf.hr

2Sveučilište J. J. Strossmayera u Osijeku, Odjel za biologiju, Ulica cara Hadrijana 8/A, 31000 Osijek

Literatura/References: 

Alm E. W.; Burke J.; Spain A. (2003.): Fecal indicator bacteria are abundant in wet sand at freshwater beaches. Water Research 37, 3978–3982.

 

Anderson K. L.; Whitlock J. E.; Harwood V.J. (2005.): Persistence and Differential Survival of Fecal Indicator Bacteria in Subtropical Waters and Sediments. Applied and Environmental Microbiology 71, 3041-3048.

 

Armah, F. A., (2014) Relationship Between Coliform Bacteria and Water Chemistry in Groundwater Within Gold Mining Environments in Ghana. Water Qual Expo Health 5(4): 183-195.

 

Cho K.H.; Pachepsky Y.A.; Kim J.H.; Guber A.K.; Shelton D.R.; Rowland R. (2010.): Release of Escherichia coli from the bottom sediment in a first-order creek: experiment and reach-specific modeling. Journal of Hydrology 391, 322-332.

 

Dekić S.; Hrenović J. (2017.): Bakteriološka analiza izvorske vode uz najpoznatija izletišta Parka prirode Medvednica. Hrvatske vode 25 (99), 13-16.

 

Ellis, R., (2004) Artificial soil microcosms: a tool for studying microbial autecology under controlled conditions. Journal of Microbiological Methods 56, 287–290.

 

Fass S.; Dincher M. L.; Reasoner D. J.; Gatel D.; Block J. C. (1996.): Fate of Escherichia coli experimentally injected in a drinking water distribution pilot system. Water Research 30, 2215–2221.

 

Kiefer L.A.; Shelton D.R.; Pachepsky Y.; Blaustein R.; Santin-Duran M. (2012.): Persistence of Escherichia coli introduced into streambed. Letters in Applied Microbiology 55, 345-353.

 

LeChevallier M. W.; McFeters G.A. (1985.): Interactions between heterotrophic plate Count Bacteria and Coliform Organisms. Applied and Environmental Microbiology 49(5), 1338-1341.

 

Matheson F.E. (2008.): Encyclopedia of Ecology, Microcosms, 545-549, National Institute of Water & Atmospheric Research, Hamilton, New Zealand.

 

Pravilnik o parametrima sukladnosti, metodama analize, monitoringu i planovima sigurnosti vode za ljudsku potrošnju te načinu vođenja registra pravnih osoba koje obavljaju djelatnost javne vodoopskrbe (NN/17).

 

Salama Y.; Chennaoui M.; Mountadar M.; Rihani M. and Assobhei O. (2013.): Evaluation of faecal coliform levels in the discharges from the city of El Jadida, Morocco.African Journal of Microbiology Research 8(2), 178-183.

 

Schuettpelz, H. D. (1969.):Fecal and total coliform tests in water quality evaluation. Department of Natural Resources, Madison, Wisconsin.

 

Zhang Q.; He X. and Yan T. (2015.): Differential Decay of Wastewater Bacteria and Change of Microbial Communities in Beach Sand and Seawater Microcosms. Environmental Science & Technology 49, 8531-8540.